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These sterile cloning cylinders are designed for isolating individual cell clones in a laboratory setting. They feature a tapered design with a wider bottom edge, ensuring a good sealing surface. To use, apply a thin layer of grease to the bottom edge, invert the cylinder over the desired clone, and incubate with trypsin or EDTA until cells detach. The cylinders are then sterilized and supplied in packs of 10, with three sizes available. Ideal for cell culture and biotechnology applications, these cloning cylinders enable researchers to isolate and grow homogeneous populations of cells for further study and experimentation.
Top product features:
Tapered design ensures a good sealing surface
Polystyrene material allows for a sterile environment
Cylindrical shape facilitates the isolation of individual cell clones
Top product applications:
Isolation of individual cell clones from a mixed cell population
Separation of cells from trypsin or EDTA solution
Collection of detached cells from inside the cloning cylinder
These cloning cylinders facilitate the isolation of individual cell clones each generated from a single cell yielding a homogenous population of cells. To isolate a clone a thin layer of grease is applied to the bottom edge of the cylinder and then inverted over the clone of choice. After adding a small volume of trypsin or edta the dish is incubated at 37°c (98.6°f) briefly until cells detach. Cells are then collected from inside the cylinder and transferred to another vessel for further growth. Ul style"margin-top0px; padding-top 5px; padding-bottom 5px;" cylinders are tapered so bottom edge is wider than the top ensuring a good sealing surface polystyrene cloning cylinders are supplied in sterile packs three sizes 4.7mm i.D. X 8mm h 6.4mm i.D. X 8mm h and 9.5mm i.D. X 11mm h packed 10 per bag one size note sterilized products are not returnable.